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il3  (R&D Systems)


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    Structured Review

    R&D Systems il3
    Il3, supplied by R&D Systems, used in various techniques. Bioz Stars score: 96/100, based on 604 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/human+il3/Recombinant+Human+M-CSF+Protein/pmc13065346-142-74-75
    Average 96 stars, based on 604 article reviews
    il3 - by Bioz Stars, 2026-10
    96/100 stars

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    Related Articles

    Plasmid Preparation:

    Article Title: Transplantation of stromal cells transduced with the human IL3 gene to stimulate hematopoiesis in human fetal bone grafts in non-obese, diabetic-severe combined immunodeficiency mice.
    Article Snippet: .. Materials and methods hu-IL3-MS5 cells We used the pCEP4 plasmid (Invitrogen, Leek, The Netherlands), a mammalian expression vector which uses the CMV enhancer/promoter for the transcription of recombinant genes.12 The coding sequence from human IL3 (R&D Systems, Minneapolis, MN, USA) was ligated to synthetic fragments containing the natural leader sequence of human IL3 and subcloned into the pCEP4 plasmid. ..

    Expressing:

    Article Title: Transplantation of stromal cells transduced with the human IL3 gene to stimulate hematopoiesis in human fetal bone grafts in non-obese, diabetic-severe combined immunodeficiency mice.
    Article Snippet: .. Materials and methods hu-IL3-MS5 cells We used the pCEP4 plasmid (Invitrogen, Leek, The Netherlands), a mammalian expression vector which uses the CMV enhancer/promoter for the transcription of recombinant genes.12 The coding sequence from human IL3 (R&D Systems, Minneapolis, MN, USA) was ligated to synthetic fragments containing the natural leader sequence of human IL3 and subcloned into the pCEP4 plasmid. ..

    Recombinant:

    Article Title: Transplantation of stromal cells transduced with the human IL3 gene to stimulate hematopoiesis in human fetal bone grafts in non-obese, diabetic-severe combined immunodeficiency mice.
    Article Snippet: .. Materials and methods hu-IL3-MS5 cells We used the pCEP4 plasmid (Invitrogen, Leek, The Netherlands), a mammalian expression vector which uses the CMV enhancer/promoter for the transcription of recombinant genes.12 The coding sequence from human IL3 (R&D Systems, Minneapolis, MN, USA) was ligated to synthetic fragments containing the natural leader sequence of human IL3 and subcloned into the pCEP4 plasmid. ..

    Article Title: Evaluation of the basophil activation test and skin prick testing for the diagnosis of sesame food allergy.
    Article Snippet: This article has been accepted for publication and undergone full peer review but has not been through the copyediting, typesetting, pagination and proofreading process, which may lead to differences between this version and the Version of Record.. Please cite this article as doi: 10.1111/cea.13174 This article is protected by copyright.. All rights reserved.

    Article Title: Inhibition of FcepsilonRI-dependent mediator release and calcium flux from human mast cells by sialic acid-binding immunoglobulin-like lectin 8 engagement.
    Article Snippet: Background: Sialic acid–binding immunoglobulin-like lectins (Siglecs) are a family of glycan-binding inhibitory receptors, and among them, Siglec-8 is selectively expressed on human eosinophils, basophils, and mast cells.. On eosinophils, Siglec-8 engagement induces apoptosis, but its function on mast cells is unknown.. Objective: We sought to study the effect of Siglec-8 engagement on human mast cell survival and mediator release responses.

    Article Title: Direct signal transduction via functional interferon-alphabeta receptors in CD34+ hematopoietic stem cells.
    Article Snippet: .. The medium was composed of 0.9% methylcellulose supplemented with 30% fetal calf serum (Dominique Deutsher, Brumath, France), 10 mg/ml transferrin, 1 mg/ml detoxified serum albumin (Sigma Chemical, St Louis, MO, USA), 2 mM glutamine (Life Technologies, Cergy-Pontoise, France), 5 × 10−5 M 2-mercaptoethanol (Merck, Darmstadt, Germany), 1.7 units/ml of recombinant human IL3, 10 units/ml of recombinant human IL-6, 3 ng/ml of recombinant human SCF (all obtained from R&D Systems), and 18.3 units/ml of recombinant human GMCSF (Genetic Institute, Cambridge, MA, USA), 2 units/ml of recombinant human EPO (Boehringer, Mannheim, Germany), Approximately 400 enriched CD34+ cells were re-suspended in 0.4 ml Iscove’s modified Dulbecco’s medium (IMDM) supplemented with 10% FCS and mixed with 2 ml of StemGem methylcellulose and 1 ml of this mixture was plated in 35- mm Petri dishes. ..

    Sequencing:

    Article Title: Transplantation of stromal cells transduced with the human IL3 gene to stimulate hematopoiesis in human fetal bone grafts in non-obese, diabetic-severe combined immunodeficiency mice.
    Article Snippet: .. Materials and methods hu-IL3-MS5 cells We used the pCEP4 plasmid (Invitrogen, Leek, The Netherlands), a mammalian expression vector which uses the CMV enhancer/promoter for the transcription of recombinant genes.12 The coding sequence from human IL3 (R&D Systems, Minneapolis, MN, USA) was ligated to synthetic fragments containing the natural leader sequence of human IL3 and subcloned into the pCEP4 plasmid. ..

    Selection:

    Article Title: Inhibition of FcepsilonRI-dependent mediator release and calcium flux from human mast cells by sialic acid-binding immunoglobulin-like lectin 8 engagement.
    Article Snippet: Background: Sialic acid–binding immunoglobulin-like lectins (Siglecs) are a family of glycan-binding inhibitory receptors, and among them, Siglec-8 is selectively expressed on human eosinophils, basophils, and mast cells.. On eosinophils, Siglec-8 engagement induces apoptosis, but its function on mast cells is unknown.. Objective: We sought to study the effect of Siglec-8 engagement on human mast cell survival and mediator release responses.

    Derivative Assay:

    Article Title: Inhibition of FcepsilonRI-dependent mediator release and calcium flux from human mast cells by sialic acid-binding immunoglobulin-like lectin 8 engagement.
    Article Snippet: Background: Sialic acid–binding immunoglobulin-like lectins (Siglecs) are a family of glycan-binding inhibitory receptors, and among them, Siglec-8 is selectively expressed on human eosinophils, basophils, and mast cells.. On eosinophils, Siglec-8 engagement induces apoptosis, but its function on mast cells is unknown.. Objective: We sought to study the effect of Siglec-8 engagement on human mast cell survival and mediator release responses.

    Modification:

    Article Title: Direct signal transduction via functional interferon-alphabeta receptors in CD34+ hematopoietic stem cells.
    Article Snippet: .. The medium was composed of 0.9% methylcellulose supplemented with 30% fetal calf serum (Dominique Deutsher, Brumath, France), 10 mg/ml transferrin, 1 mg/ml detoxified serum albumin (Sigma Chemical, St Louis, MO, USA), 2 mM glutamine (Life Technologies, Cergy-Pontoise, France), 5 × 10−5 M 2-mercaptoethanol (Merck, Darmstadt, Germany), 1.7 units/ml of recombinant human IL3, 10 units/ml of recombinant human IL-6, 3 ng/ml of recombinant human SCF (all obtained from R&D Systems), and 18.3 units/ml of recombinant human GMCSF (Genetic Institute, Cambridge, MA, USA), 2 units/ml of recombinant human EPO (Boehringer, Mannheim, Germany), Approximately 400 enriched CD34+ cells were re-suspended in 0.4 ml Iscove’s modified Dulbecco’s medium (IMDM) supplemented with 10% FCS and mixed with 2 ml of StemGem methylcellulose and 1 ml of this mixture was plated in 35- mm Petri dishes. ..



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